Japanese scientists design bioluminescent molecules hundreds of times brighter in deep tissues to image the cells from outside the body. The bioengineered light source was then used to track cancer cells in mice and brain-cell activity in monkeys, but its applications extend beyond the lab.
Tokyo (ISJ) Japanese scientists have designed bioluminescent molecules hundreds of times brighter in deep tissues to image cells from outside the body. The bioengineered light source was then used to track cancer cells in mice and brain-cell activity in monkeys, but its applications extend beyond the lab.
Bioluminescence is the result of a partnership: an enzyme-in this case luciferase derived from fireflies-catalyzes the substrate D-luciferin, creating a green-yellow glow in the process. There has been considerable research to make this process more efficient. For example, swapping out luciferin for synthetic analogs and improving the rate of catalysis.
Researchers led by Atsushi Miyawaki of the RIKEN Brain Science Institute sought to go further, refining both ingredients to create AkaBLI – a completely bioengineered bioluminescence system for in vivo use. The University of Electro-Communications, Tokyo Institute of Technology and Kyoto University were collaborating partners in this study.
Based on previous work, the researchers knew that a synthetic luciferin called AkaLumine-HCl is able to penetrate the blood-brain barrier and produce a reddish light that is more easily seen in body tissues. It was not very compatible with the natural luciferase, however, so they successively mutated the enzyme to improve pairing with AkaLumine-HCl. The resulting Akaluc protein is both a more efficient catalyst for the substrate and more abundantly expressed by cells.
In the mouse brain, this combination of Akaluc catalyzing AkaLumine-HCl, dubbed AkaBLI, resulted in a bioluminescence signal 1000 times stronger than that from the natural luciferase-luciferin reaction. Elsewhere in the body, just one or two glowing cells were clearly visible from within the mouse lung, something that could be useful for monitoring transplanted cells.
Bioluminescence can be introduced easily and voluntarily by including AkaBLI in animals’ drinking water, which gives the most persistent glow, although injecting the molecules yielded greater intensity.
“The fundamental improvement, though, is the practical applicability for in vivo physiological studies,” says Miyawaki.
With AkaBLI, how brain activity and structures change with behaviour can be directly observed over time. In an experiment in which mice were exposed to familiar and new cage environments, the same neurons in the hippocampus could be recorded over multiple days.
“This is the first time such a small ensemble of a few dozen deep neurons related to a specific learning behaviour can be visualized non-invasively,” said Miyawaki.
And in a marmoset monkey, the researchers were able to track deep-brain neurons for more than a year using AkaBLI. The potential for this kind of stable and long-lasting bioluminescence for understanding neural circuitry during natural behaviors, observed Miyawaki, is intense.
